Thymosin Beta 4 (TB500)

A single-component research material supplied for controlled research environments. Suitable for studies involving peptide characterization and method development in model systems.

Composition

• Thymosin Beta 4 (TB-500)
• Appearance: Lyophilized powder in a sealed research vial

Research Focus (non-clinical)

• Peptide identity and purity assessment via HPLC/LC-MS
• Assay development and calibration for quantitative analysis
• Chromatographic method optimization for impurity profiling and peak resolution
• Stability characterization of a lyophilized peptide under laboratory storage conditions

For qualified research professionals and institutional laboratories. Not for human use.

Documentation & Quality Assurance

Each lot is sourced through our verified global supply chain with emphasis on traceability and quality control. We work diligently to obtain and maintain third-party analytical reports (HPLC/LC-MS) and Certificates of Analysis for each batch, as part of our ongoing quality process. These documents are reviewed internally and displayed as they become available. Independent third-party testing is also performed on select lots to confirm identity, purity, and alignment with our internal specifications.

Important Notice

This product is intended for laboratory research use only. It is not intended for human or veterinary use, and must not be used for diagnostic, therapeutic, or clinical purposes.
This material is not a drug, medical device, or dietary supplement, and has not been evaluated by the U.S. Food and Drug Administration.

Quality & Manufacturing

All materials are sourced from carefully vetted domestic and international manufacturing partners who follow quality systems consistent with ISO and cGMP principles. Each supplier is reviewed for reliability, documentation integrity, and transparency in testing.

We require a verified purity of 99% or higher and perform independent third-party spot testing to confirm that select lots meet our internal standards for identity, purity, and composition. Where available, endotoxin testing results are included on Certificates of Analysis to verify laboratory purity; their inclusion is for research quality assessment only and does not imply suitability for human or veterinary use.

All research materials are sealed for integrity and packaged for stability during storage and transport from manufacturing through final delivery.

What It Is

Thymosin beta-4 (Tbeta4) is a ubiquitous 43 amino acid, 5 kDa G-actin-sequestering polypeptide belonging to the beta-thymosin family; TB-500 is a synthetic peptide corresponding to the N-acetylated heptapeptide LKKTETQ, derived from the actin-binding domain of thymosin beta-4.

Research Background

Thymosin beta-4 and its actin-binding domain fragment have been examined across a range of in vitro cell culture systems, ex vivo tissue preparations, and animal models. In vitro studies have employed human umbilical vein endothelial cells (HUVECs), adult human cardiac myocytes, adult human cardiac fibroblasts, and chick aortic arch preparations to probe cellular responses at the molecular level. Animal research has included murine myocardial infarction models (permanent left anterior descending coronary artery ligation in C57BL/6J mice) in which systemic peptide administration was studied. Separately, pharmacokinetic and metabolite profiling of TB-500 — the synthetic LKKTETQ fragment — has been conducted in an equine model using liquid chromatography-mass spectrometry analysis of urine and plasma collected after a single administered dose, representing the first identification of TB-500 and its metabolites in post-administration biological samples from horses.

Observed Areas of Interest in Research

Structural studies using chemical cross-linking have shown that thymosin beta-4 contacts both the barbed and pointed ends of an actin monomer in an extended conformation, with lysine residues at positions 3 and 18 cross-linking to actin subdomains 3 and 1 respectively, and Lys-38 contacting subdomain 2 at the pointed end — a geometry proposed to sterically block actin polymerization and maintain a cellular reservoir of unpolymerized G-actin. Research on the seven-amino-acid actin-binding motif (LKKTETQ) has investigated its role in endothelial cell migration and vessel sprouting in vitro, with findings indicating that peptides lacking any portion of this motif were inactive in those assays and that the adhesion and sprouting activity observed was inhibited by the addition of exogenous soluble actin. Studies in a murine cardiac injury model have examined the peptide's relationship to Rho-associated protein kinase 1 (ROCK1) signaling, observing selective downregulation of ROCK1 protein in infarcted and remote cardiac tissue, concurrent upregulation of miR139-5p (a microRNA targeting ROCK1), and cell-type-dependent changes in actin filament organization and alpha-smooth muscle actin expression across cardiac fibroblasts, myocytes, and endothelial cells under both normoxic and hypoxic conditions.

Research Notes

Purity verified by third-party laboratory analysis (Certificate of Analysis available on request). Supplied strictly for in-vitro and laboratory research use only. Not for human consumption.

All information above is provided for research and educational reference only and summarizes findings from published scientific literature (predominantly in-vitro and animal models). It does not describe outcomes in humans. This product is not a drug, dietary supplement, or article intended for human or veterinary use.

All products from Blitz Health are manufactured using a lyophilization (freeze-drying) process. This method is designed to maintain product integrity and allows vials to remain stable during shipping for approximately 3–4 months.

Once a vial is reconstituted with bacteriostatic water, it should be stored in the refrigerator to help maintain stability. Under these conditions, reconstituted material is generally considered stable for up to 30 days.

Lyophilization is a dehydration technique in which compounds are frozen and then exposed to low pressure. This causes the water in the vial to sublimate directly from solid to gas, leaving behind a stable, crystalline white structure. This powder can be kept at room temperature until reconstitution.

Upon receipt, products should be stored away from heat and light. For short-term use, refrigeration at approximately 4°C (39°F) is suitable. For long-term storage (several months to years), vials may be placed in a freezer at approximately -80°C (-112°F). Freezing is the preferred method for preserving product stability over extended periods.

⚠️ Important Notice:
These products are intended for research use only. Not for human consumption.