4X Blend Methionine (15mg) / Choline Chloride (50mg) / Carnitine (50mg) / Dexpanthenol (5mg)

A multi-component research material supplied for controlled research environments. This 4X blend combines Methionine, Choline Chloride, Carnitine, and Dexpanthenol in a single research vial, supporting advanced studies of amino acid and lipid metabolism in model systems.

Composition

• Pre-formulated liquid solution
• Total concentration: 120mg/mL — 10mL vial
• Choline Chloride 50mg/mL
• L-Carnitine 50mg/mL
• L-Methionine 15mg/mL
• Dexpanthenol 5mg/mL
• Appearance: Fully constituted liquid solution in a sealed 10 mL research vial
• Storage: Keep out of light, tightly closed, dry. Store between 15–25°C (room temp) or 2–8°C (refrigerated). Do not freeze.

Research Focus (non-clinical)

• Investigation of methylation pathways and lipid metabolism
• Studies examining carnitine-dependent fatty acid transport and energy production
• Assay development for quantifying methionine, choline, and carnitine utilization
• Stability and compatibility testing of multi-component nutrient blends in solution

For qualified research professionals and institutional laboratories. Not for human use.

Documentation & Quality Assurance

Each lot is sourced through our verified global supply chain with emphasis on traceability and quality control. We work diligently to obtain and maintain third-party analytical reports (HPLC/LC-MS) and Certificates of Analysis for each batch, as part of our ongoing quality process. These documents are reviewed internally and displayed as they become available. Independent third-party testing is also performed on select lots to confirm identity, purity, and alignment with our internal specifications.

Important Notice

This product is intended for laboratory research use only. It is not intended for human or veterinary use, and must not be used for diagnostic, therapeutic, or clinical purposes.
This material is not a drug, medical device, or dietary supplement, and has not been evaluated by the U.S. Food and Drug Administration.

Quality & Manufacturing

All materials are sourced from carefully vetted domestic and international manufacturing partners who follow quality systems consistent with ISO and cGMP principles. Each supplier is reviewed for reliability, documentation integrity, and transparency in testing.

We require a verified purity of 99% or higher and perform independent third-party spot testing to confirm that select lots meet our internal standards for identity, purity, and composition. Where available, endotoxin testing results are included on Certificates of Analysis to verify laboratory purity; their inclusion is for research quality assessment only and does not imply suitability for human or veterinary use.

All research materials are sealed for integrity and packaged for stability during storage and transport from manufacturing through final delivery.

What It Is

A research compound comprising four biochemically distinct molecules — L-methionine (an aliphatic, sulfur-containing essential amino acid), choline chloride (the chloride salt form of the quaternary ammonium compound choline, a required dietary nutrient and methyl donor), L-carnitine (a trimethylated amino acid derivative), and dexpanthenol (the dextrorotatory alcohol form of pantothenic acid, classified as a provitamin B5 precursor) — formulated together for investigational use in research contexts.

Research Background

L-Methionine has been examined in review studies across mammalian models as a precursor in the synthesis of homocysteine, cysteine, creatine, and carnitine, with investigators exploring its roles in oxidative stress regulation, innate immune signaling, and lipid metabolism. Choline chloride has been investigated in rodent models — including agouti and axin(Fu) mice — and in vitro hepatocyte systems for its participation in one-carbon (methyl-carbon) metabolism and epigenetic gene regulation via the BHMT-dependent pathway linking choline to SAM and S-adenosylhomocysteine concentrations. L-Carnitine's essentiality for mitochondrial fatty acid catabolism has been characterised through in vitro fibroblast uptake assays, clinical newborn-screening analyses, and molecular studies of the SLC22A5-encoded OCTN2 transporter, with primary carnitine deficiency models used to delineate transport kinetics. Dexpanthenol has been studied in in vitro fibroblast proliferation models, murine acute lung injury preparations, and randomised controlled trials examining skin barrier restoration, with its bioactivity attributed to its enzymatic oxidation to pantothenic acid and subsequent incorporation into coenzyme A.

Observed Areas of Interest in Research

Researchers investigating L-methionine have focused on its role as a precursor to S-adenosylmethionine (SAM) — the principal biological methyl-group donor — and on methionine sulfoxide reductase activity and glutathione biosynthesis as mechanisms by which the amino acid interfaces with cellular redox homeostasis. For choline chloride, studied pathways center on the betaine homocysteine methyltransferase (BHMT) enzyme, through which choline-derived betaine regulates SAM/S-adenosylhomocysteine ratios and downstream DNA methyltransferase (DNMT) and histone methyltransferase (HMT) activity, placing choline at the intersection of one-carbon metabolism and epigenetic regulation. L-Carnitine research has concentrated on the carnitine shuttle system — comprising carnitine palmitoyltransferases CPT1 and CPT2 and the carnitine acyl-carnitine translocase (CACT) — through which long-chain acyl-CoA species are translocated across the inner mitochondrial membrane to enter beta-oxidation, a pathway studied particularly in cardiac, hepatic, and skeletal muscle tissues. Dexpanthenol investigation has mapped its conversion pathway to pantothenic acid and then to coenzyme A, a cofactor characterised as participating in numerous biochemical reactions including fatty acid synthesis and oxidation, intermediary metabolism, and neurotransmitter synthesis.

Research Notes

Purity verified by third-party laboratory analysis (Certificate of Analysis available on request). Supplied strictly for in-vitro and laboratory research use only. Not for human consumption.

All information above is provided for research and educational reference only and summarizes findings from published scientific literature (predominantly in-vitro and animal models). It does not describe outcomes in humans. This product is not a drug, dietary supplement, or article intended for human or veterinary use.

All products from Blitz Health are manufactured using a lyophilization (freeze-drying) process. This method is designed to maintain product integrity and allows vials to remain stable during shipping for approximately 3–4 months.

Once a vial is reconstituted with bacteriostatic water, it should be stored in the refrigerator to help maintain stability. Under these conditions, reconstituted material is generally considered stable for up to 30 days.

Lyophilization is a dehydration technique in which compounds are frozen and then exposed to low pressure. This causes the water in the vial to sublimate directly from solid to gas, leaving behind a stable, crystalline white structure. This powder can be kept at room temperature until reconstitution.

Upon receipt, products should be stored away from heat and light. For short-term use, refrigeration at approximately 4°C (39°F) is suitable. For long-term storage (several months to years), vials may be placed in a freezer at approximately -80°C (-112°F). Freezing is the preferred method for preserving product stability over extended periods.

⚠️ Important Notice:
These products are intended for research use only. Not for human consumption.