SS-31

A single-component research material supplied for controlled research environments. Suitable for studies involving mitochondrial-targeting peptide characterization and method development in model systems.

Composition

• SS-31
• Appearance: Lyophilized powder in a sealed research vial

Research Focus (non-clinical)

• Peptide identity and purity assessment via HPLC/LC-MS
• Assay development and calibration for quantitative analysis
• Chromatographic method optimization for impurity profiling and peak resolution
• Stability characterization of a lyophilized peptide under laboratory storage conditions

For qualified research professionals and institutional laboratories. Not for human use.

Documentation & Quality Assurance

Each lot is sourced through our verified global supply chain with emphasis on traceability and quality control. We work diligently to obtain and maintain third-party analytical reports (HPLC/LC-MS) and Certificates of Analysis for each batch, as part of our ongoing quality process. These documents are reviewed internally and displayed as they become available. Independent third-party testing is also performed on select lots to confirm identity, purity, and alignment with our internal specifications.

Important Notice

This product is intended for laboratory research use only. It is not intended for human or veterinary use, and must not be used for diagnostic, therapeutic, or clinical purposes.
This material is not a drug, medical device, or dietary supplement, and has not been evaluated by the U.S. Food and Drug Administration.

Quality & Manufacturing

All materials are sourced from carefully vetted domestic and international manufacturing partners who follow quality systems consistent with ISO and cGMP principles. Each supplier is reviewed for reliability, documentation integrity, and transparency in testing.

We require a verified purity of 99% or higher and perform independent third-party spot testing to confirm that select lots meet our internal standards for identity, purity, and composition. Where available, endotoxin testing results are included on Certificates of Analysis to verify laboratory purity; their inclusion is for research quality assessment only and does not imply suitability for human or veterinary use.

All research materials are sealed for integrity and packaged for stability during storage and transport from manufacturing through final delivery.

What It Is

SS-31 (Elamipretide; MTP-131) is a synthetic, cell-permeable tetrapeptide with the sequence D-Arg-Dmt-Lys-Phe-NH2 — where Dmt denotes 2',6'-dimethyltyrosine — characterized by alternating cationic and aromatic residues that confer selective localization to the inner mitochondrial membrane through electrostatic and hydrophobic association with the phospholipid cardiolipin.

Research Background

SS-31/Elamipretide has been examined in numerous peer-reviewed publications spanning cell-based assays, isolated mitochondria preparations, and multiple animal species, as well as early-phase human clinical trials. In vitro work has employed models including permeabilized cardiomyocytes, iPSC-derived cardiomyocytes from patients with mitochondrial cardiomyopathy, neuroblastoma cell lines, and isolated mitochondria from human and animal cardiac tissue. Animal investigations have utilized rat bilateral renal ischemia-reperfusion models, dog microembolization-induced heart failure models, tafazzin-knockdown mouse models of Barth syndrome, aged mouse models, and swine atherosclerotic renovascular models. Clinical research includes completed Phase 2/3 trials (TAZPOWER, MMPOWER-3, PROGRESS-HF, ReCLAIM-2, EMBRACE-STEMI), with investigators noting that overall clinical results have been mixed and that preclinical findings have not uniformly translated to human endpoints.

Observed Areas of Interest in Research

Researchers have concentrated on the compound's interaction with cardiolipin (CL) in the inner mitochondrial membrane: binding studies using fluorescence spectroscopy and circular dichroism reported a binding affinity (KD approximately 1.87 µM) through both electrostatic contacts between the basic residues (D-Arg, Lys) and CL's anionic phosphate head groups and hydrophobic contacts within the acyl chain region. Investigations have further characterized how this association is proposed to influence the assembly and stability of electron transport chain (ETC) supercomplexes (Complexes I, III, and IV), to modulate coupling of supercomplex-associated enzyme complexes, and to modulate adenine nucleotide translocator (ANT1)-mediated ADP uptake. Studies in ischemia-reperfusion models have examined effects on cytochrome c peroxidase activity — specifically whether the SS-31/CL complex limits cardiolipin peroxidation and thereby preserves cytochrome c's electron-shuttling function toward Complex IV — as well as effects on mitochondrial cristae morphology, mitochondrial membrane potential, ATP recovery kinetics, reactive oxygen species (ROS) emission, and mitochondrial permeability transition pore (mPTP) opening. These mechanisms have been characterized primarily in preclinical cell and animal models; findings from early clinical trials are preliminary and, per investigators, remain variable.

Research Notes

Purity verified by third-party laboratory analysis (Certificate of Analysis available on request). Supplied strictly for in-vitro and laboratory research use only. Not for human consumption.

All information above is provided for research and educational reference only and summarizes findings from published scientific literature (predominantly in-vitro and animal models). It does not describe outcomes in humans. This product is not a drug, dietary supplement, or article intended for human or veterinary use.

All products from Blitz Health are manufactured using a lyophilization (freeze-drying) process. This method is designed to maintain product integrity and allows vials to remain stable during shipping for approximately 3–4 months.

Once a vial is reconstituted with bacteriostatic water, it should be stored in the refrigerator to help maintain stability. Under these conditions, reconstituted material is generally considered stable for up to 30 days.

Lyophilization is a dehydration technique in which compounds are frozen and then exposed to low pressure. This causes the water in the vial to sublimate directly from solid to gas, leaving behind a stable, crystalline white structure. This powder can be kept at room temperature until reconstitution.

Upon receipt, products should be stored away from heat and light. For short-term use, refrigeration at approximately 4°C (39°F) is suitable. For long-term storage (several months to years), vials may be placed in a freezer at approximately -80°C (-112°F). Freezing is the preferred method for preserving product stability over extended periods.

⚠️ Important Notice:
These products are intended for research use only. Not for human consumption.