NAD+

A single-component research material supplied for controlled research environments. Suitable for studies involving nucleotide cofactor characterization and method development in model systems.

Composition

• NAD+
• Format: Synthetic
• Fill Volume: 6 mL vial
• Appearance: Sterile solution in a sealed research vial

Research Focus (non-clinical)

• Identity and purity assessment via HPLC/LC-MS and UV-Vis quantitation
• Assay development and calibration for enzymatic cycling or cell-free systems
• Chromatographic method optimization for impurity/degradation profiling (e.g., NADH/NADP)
• Stability characterization under laboratory storage conditions (temperature, pH, light)

For qualified research professionals and institutional laboratories. Not for human use.

Documentation & Quality Assurance

Each lot is sourced through our verified global supply chain with emphasis on traceability and quality control. We work diligently to obtain and maintain third-party analytical reports (HPLC/LC-MS) and Certificates of Analysis for each batch, as part of our ongoing quality process. These documents are reviewed internally and displayed as they become available. Independent third-party testing is also performed on select lots to confirm identity, purity, and alignment with our internal specifications.

Important Notice

This product is intended for laboratory research use only. It is not intended for human or veterinary use, and must not be used for diagnostic, therapeutic, or clinical purposes.
This material is not a drug, medical device, or dietary supplement, and has not been evaluated by the U.S. Food and Drug Administration.

Quality & Manufacturing

All materials are sourced from carefully vetted domestic and international manufacturing partners who follow quality systems consistent with ISO and cGMP principles. Each supplier is reviewed for reliability, documentation integrity, and transparency in testing.

We require a verified purity of 99% or higher and perform independent third-party spot testing to confirm that select lots meet our internal standards for identity, purity, and composition. Where available, endotoxin testing results are included on Certificates of Analysis to verify laboratory purity; their inclusion is for research quality assessment only and does not imply suitability for human or veterinary use.

All research materials are sealed for integrity and packaged for stability during storage and transport from manufacturing through final delivery.

What It Is

Nicotinamide adenine dinucleotide (NAD+) is an endogenous pyridine dinucleotide coenzyme formed by the covalent linkage of nicotinamide mononucleotide (NMN) and adenosine monophosphate, present in all living cells in oxidized (NAD+) and reduced (NADH) interconvertible forms.

Research Background

NAD+ has been investigated across a broad spectrum of experimental systems, including in vitro cell-culture models (primary neuronal cultures, astrocyte cocultures, and established cell lines), invertebrate models (Saccharomyces cerevisiae, Caenorhabditis elegans, and Drosophila melanogaster), and rodent models encompassing transgenic strains, knockout lines, and pharmacological challenge protocols. Human observational and intervention studies have examined NAD+ in the context of aging, neurodegeneration, and metabolic conditions, as documented in review literature published on PMC. In vivo imaging data cited in the peer-reviewed literature has assessed NAD+ levels and mitochondrial function in brain tissue of aged human subjects, representing early-stage translational inquiry rather than established clinical outcomes.

Observed Areas of Interest in Research

Researchers have studied NAD+ as a hydride-transfer coenzyme central to oxidative phosphorylation, in which the reversible interconversion between NAD+ and NADH serves as an electron carrier within metabolic pathways. Beyond redox chemistry, NAD+ is consumed as a co-substrate by three principal enzyme families: poly(ADP-ribose) polymerases (PARPs), which hydrolyze NAD+ to transfer ADP-ribose moieties onto target proteins in response to DNA strand breaks; the sirtuin family (SIRT1–SIRT7), NAD+-dependent deacylases that remove acetyl groups from lysine residues of substrate proteins and have been linked in model systems to pathways including mitochondrial biogenesis via PGC-1α and antioxidant responses via SOD2; and ADP-ribosyl cyclases (CD38/CD157), multifunctional ectoenzymes that catalyze the glycohydrolysis of NAD+ to generate cyclic ADP-ribose and other signaling metabolites. The regulation of intracellular NAD+ pools through biosynthetic routes—including the NAMPT-dependent salvage pathway, the kynurenine de novo pathway from tryptophan, and the Preiss-Handler pathway—has also been an active area of mechanistic investigation in both cellular and whole-organism models.

Research Notes

Purity verified by third-party laboratory analysis (Certificate of Analysis available on request). Supplied strictly for in-vitro and laboratory research use only. Not for human consumption.

All information above is provided for research and educational reference only and summarizes findings from published scientific literature (predominantly in-vitro and animal models). It does not describe outcomes in humans. This product is not a drug, dietary supplement, or article intended for human or veterinary use.

All products from Blitz Health are manufactured using a lyophilization (freeze-drying) process. This method is designed to maintain product integrity and allows vials to remain stable during shipping for approximately 3–4 months.

Once a vial is reconstituted with bacteriostatic water, it should be stored in the refrigerator to help maintain stability. Under these conditions, reconstituted material is generally considered stable for up to 30 days.

Lyophilization is a dehydration technique in which compounds are frozen and then exposed to low pressure. This causes the water in the vial to sublimate directly from solid to gas, leaving behind a stable, crystalline white structure. This powder can be kept at room temperature until reconstitution.

Upon receipt, products should be stored away from heat and light. For short-term use, refrigeration at approximately 4°C (39°F) is suitable. For long-term storage (several months to years), vials may be placed in a freezer at approximately -80°C (-112°F). Freezing is the preferred method for preserving product stability over extended periods.

⚠️ Important Notice:
These products are intended for research use only. Not for human consumption.